blot n. 1.墨污,墨漬,污點(diǎn),污斑。 2.瑕疵;恥辱,污名。 3.〔古語(yǔ)〕涂去,抹去。 a blot on her past [her character] 她歷史[品格]上的污點(diǎn)。 drop an ink blot on an envelope 信封玷上了一塊墨污。 vt. (-tt-) 1.用(墨水等)弄臟,涂污。 2.涂去,抹掉(out)。 3.用吸墨紙吸干。 4.遮蔽(風(fēng)景等),遮暗,使昏暗。 5.玷污,損害(名譽(yù)等)。 6.〔美俚〕殺死;亂涂,亂寫(xiě);〔詩(shī)〕擦掉。 The sun was blotted by the moon. 月亮遮暗了太陽(yáng),日蝕。 blot the wet pane 擦干潮濕的窗玻璃。 blotting pad 吸墨紙滾臺(tái)。 vi. 1.(墨水)滲開(kāi);造成污漬。 2.(紙等)玷上墨污;(吸墨紙)吸掉墨水。 This pen blots. 這枝筆漏墨水。 This paper blots easily. 這種紙容易吸水。 blot one's copybook 損壞自己名譽(yù)。 blot out 1. 涂去(文字)。 2. 遮掉(風(fēng)景等),遮暗。 3. 〔美俚〕消滅,殺掉;毀掉。 n. 1.(十五子棋中)易被吃掉的孤立棋子。 2.弱點(diǎn)。 hit a blot 吃去弱子,突破弱點(diǎn)。
dot n. 多特 〔Dorothea 的昵稱〕。 n. 1.點(diǎn);圓點(diǎn);句點(diǎn);【音樂(lè)】附點(diǎn)〔音符后的一點(diǎn),表示延長(zhǎng)1/2拍〕。 2.一點(diǎn)點(diǎn)大的東西,小片,少量,小個(gè)子。 3.【數(shù)學(xué)】小數(shù)點(diǎn);相乘的符號(hào)。 There are dots of soot on the window sill. 窗臺(tái)上有一點(diǎn)煙灰。 Put a dot on [over] the i. 給字母 i 加上一點(diǎn)。 a dot of butter 一點(diǎn)兒奶油。 a mere dot of a child 小小的孩子。 in the year dot 〔口語(yǔ)〕老早以前。 off one's dot 〔俚語(yǔ)〕傻頭傻腦的;發(fā)瘋。 on the dot 〔口語(yǔ)〕按時(shí);準(zhǔn)時(shí)。 put dots on 〔口語(yǔ)〕使倦怠[煩悶]。 to a dot 〔美國(guó)〕完全,全部 (be correct to a dot 完全正確)。 to the dot of an i 一絲不茍地。 vt. (-tt-) 1.打點(diǎn)于;星羅棋布于,點(diǎn)綴;用點(diǎn)線表示。 2.〔俚語(yǔ)〕敲,打。 Dot your i's and j's. 寫(xiě)字時(shí)不要忘記在i和j字上打一點(diǎn)。 the sea dotted with ships 點(diǎn)綴著船只的海洋。 Trees dot the landscape. 樹(shù)木點(diǎn)綴著景色。 Stars dot the sky. 星星綴滿天空。 a dotted note 【音樂(lè)】附點(diǎn)音符。 dot a line across the page 橫貫書(shū)頁(yè)劃一條虛線。 dot sb. in the eye 一拳打到某人眼上。 a dotted line 虛線。 dot and carry one 〔做加法時(shí)〕逢十進(jìn)位〔兒童用語(yǔ)〕。 dot and go one 1. = dot and carry one. 2.n. (拄著丁字杖走的)瘸子;一瘸一拐的步行聲。 3. 一瘸一拐的[地]。 dot down 暫且記下來(lái)。 dot one's i's and cross one's t's 打 i 的點(diǎn)畫(huà) t 的橫線;一筆不茍,詳述。 sign on the dotted line 〔美國(guó)〕在(信封、文件等留出供署名的)虛線上簽名;〔比喻〕全盤(pán)接受。 n. 【法律】嫁妝,嫁資,妝奩。 adj. -al
Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium 從培養(yǎng)基中可檢測(cè)到可溶性抗人絨毛膜促性腺激素的單鏈抗體。
Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city 最后,用地高辛隨機(jī)引物法標(biāo)記成momp基因核酸探針,斑點(diǎn)雜交檢測(cè)衣原體基因組dna ,靈敏度可達(dá)10pg ,且不能檢出其它病原體的核酸。將核酸探針?lè)ㄅc補(bǔ)體結(jié)合反應(yīng)法對(duì)衣原體感染的診斷進(jìn)行比較,初步證明該探針具有較高的敏感性與較強(qiáng)的特異性。
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn 用攜帶有幾丁質(zhì)酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過(guò)農(nóng)桿菌介導(dǎo)法和基因槍轉(zhuǎn)化法轉(zhuǎn)化龍牙百合,經(jīng)pcr和點(diǎn)雜交檢測(cè)證明外源基因已經(jīng)整合到植物染色體中。同時(shí)對(duì)農(nóng)桿菌介導(dǎo)法和基因槍法進(jìn)行比較,發(fā)現(xiàn)農(nóng)桿菌介導(dǎo)法的轉(zhuǎn)化率為16 . 7 ,基因槍法的轉(zhuǎn)化率為50 ,因此可能基因槍轉(zhuǎn)化法更適于龍牙百合的遺傳轉(zhuǎn)化。
Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 % 本研究中首次對(duì)iltv - nm98a株的tk基因進(jìn)行了克隆和序列分析,結(jié)果表明: iltv - nm98a株tk基因的核苷酸序列與已發(fā)表的iltvtk基因的核苷酸序列具有高度的同源性,兩者之間僅相差4個(gè)核苷酸,同源性高達(dá)99 . 7 ,從而證實(shí)了iltvtk基因是高度保守的,為iltvtk基因核酸探針的制備提供了有力的依據(jù)。